Test system for detection of Bacillus Anthracis using polymerase chain reaction (PCR)

The test system is designed for detection and identification of Bacillus anthracis containing the capsule gene Сар В. This test system allows detection of the vegetative form of the capsule-forming B.anthracis at concentrations upwards of 103 cells per ml in microbiological cultures and liquid samples from the environment and at concentrations upwards of 2х103 cells/g of tissue in blood, spleen, heart or liver samples obtained from sick or dead animals. The kit contains sufficient reagents to perform 50 tests.

The assay consists of the isolation of the total DNA, PCR amplification of a specific DNA fragment and agarose gel electrophoresis.

• набор для выделения ДНК (набор 1);
• набор для выявления Bacillus anthracis методом PCR (набор 2);
• набор для проведения электрофореза (набор 3).

Тест-система рассчитана на проведение 50 анализов.

От 6 до 8 часов (в зависимости от количества проб и используемого оборудования).

Bacillus anthracis can be detected using PCR. The specific DNA fragment is amplified millions of times, which allows visualization of the results using electrophoresis in agarose gel.

The presence or absence of DNA of the Siberian plague causative agent in the sample is assessed by electrophoresis.

The test system allows differentiation between Bacillus anthracis and the so called non-typical bacteria, not pathogenic in man and animals.

Kit 2 is stored at a temperature between −18 and −20°C, kits 1 and 3 are stored at a temperature between 2 and 6°C.

12 months.