Test system for detection of pathogenic leptospirae using PCR

The test system is designed for detection of the 15 most common serovars of Leptospira interrogans in blood and urine samples from sick animals, pathological material from dead or forcedly killed animals and in infected cell cultures.
The kit contains sufficient reagents to perform 50 tests. The assay consists of the isolation of the total DNA, PCR amplification of a specific DNA fragment and agarose gel electrophoresis.

• набор для выделения ДНК (набор 1);
• набор для определения ДНК патогенных лептоспир методом PCR (набор 2);
• набор для проведения электрофореза (набор 3).

Тест-система рассчитана на проведение 50 анализов.

Total assay run time:

От 8 до 16 часов (в зависимости от количества проб и используемого оборудования).

Leptospira interrogans can be detected using PCR. The specific DNA fragment is amplified millions of times, which allows visualization of the results using agarose gel electrophoresis.

Among the advantages of the test system is the capacity to detect the causative agent on the second day after infection. The test system allows differentiation between the pathogenic species of Leptospira and the saprophyte species L. biflexa, non-pathogenic in man and animals.

Kit 2 is stored at a temperature between −18 and −20°C, kits 1 and 3 are stored at a temperature between 2 and 6°С.

12 months.