Test system for detection and differentiation of influenza virus of subtype Н5 using real time PCR

The test system is designed for detection of influenza A virus of subtype Н5 in blood and tissue samples from sick animals and in infected cell cultures.

The kit contains sufficient reagents to perform 50 tests.

The assay consists of the isolation of the total RNA, reverse transcription reaction and amplification (RT PCR) of the specific fragment using real time PCR.

• набор для выделения РНК (набор 1);
• набор для выявления РНК вируса гриппа А подтипа Н5 методом PCR в реальном времени (набор 2).

Тест-система рассчитана на проведение 50 анализов.

От 4 до 5 часов.

Real time PCR allows simultaneous amplification and quantification of PCR products.

The amplified fragment of the viral DNA can be detected using a molecular beacon (a short chemically synthesised single-stranded oligonucleotide 24 b.p. long) complementary to the inner part of the amplified fragment of the virus that carries a fluorescent label at the 5'-end and a fluorescence quencher at the 3'-end. The beacon undergoes a conformational reorganization in the course of PCR, as the fragment of the virus is amplified and forms a hybrid complex with the beacon, spatially separating the fluorescent label and quencher, thus exposing the fluorescent label. By measuring the fluorescence intensity, the results of PCR amplification can be visualized without gel electrophoresis.

A major advantage is that reaction tubes stay closed during fluorescence measurements, which prevents contamination of the PCR products.

The test system allows detection of influenza A virus of subtype Н5 and differentiation between subtype H5 virus and other influenza A subtype viruses, paramyxoviruses and other virus families.

Kit 2 is stored at a temperature between −18 and −20°С, kit 1 is stored at a temperature between 2 and 6°С.

12 months.