Test system for detection and differentiation of influenza virus of subtypes Н5 and Н7 using PCR

The test system is designed for detection of influenza A virus of subtypes Н5 and Н7 in blood and tissue samples from sick animals and birds and in infected cell cultures.

The kit contains sufficient reagents to perform 50 tests.

The assay consists of the isolation of the total RNA, reverse transcription reaction and amplification (RT PCR) of the specific fragment using PCR, reamplification and agarose gel electrophoresis.

• набор для выделения РНК (набор 1);
• набор для выявления и дифференциации РНК вирусов гриппа А подтипов Н5 и Н7 методом PCR (набор 2);
• набор для проведения электрофореза (набор 3).

Тест-система рассчитана на проведение 50 анализов с двумя парами праймеров.

От 8 до 16 часов (в зависимости от количества проб и используемого оборудования).

Influenza A virus of subtypes H5 and H7 is detected using RT PCR, whereby cDNA is synthesized from RNA and its specific fragment is amplified millions of times, which allows visualization of the results using agarose gel electrophoresis.

The test system is designed so that only the subtypes H5 and H7 are detected in the course of RT PCR. The differentiation between the two subtypes is accomplished in the course of reamplification, when two specific pairs of primers are used for each subtype. The resulting PCR fragments differ in length, which further facilitates the differentiation.

Kit 2 is stored at a temperature between −18 and −20°С, kits 1 and 3 are stored at a temperature between 2 and 6°С.

12 months.