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Kit for detection of antibodies to porcine transmissive gastroenteritis virus using ELISA ("TGE-SEROTEST")

Kit for detection of antibodies to porcine transmissive gastroenteritis virus using ELISA (

a plate for immunological assays pre-coated with TGE antigen;
positive and negative control sera;
horseradish peroxidase labelled anti-swine IgG;
non-specific chemical components.

Description:

The kit is designed for detection of antibodies to porcine transmissive gastroenteritis virus (TGE) in serum using an immunoenzyme assay for diagnostics of TGE in unvaccinated animals and for evaluation of Immunity status after vaccination.

The kit contains sufficient reagents to test 46 samples (2 repeats) and 2 control samples (2 sera).

The design of the kit allows fractional utilization of components.

The kit includes:

Total assay run time:

2,5 - 3 hours.

Principle of the action:

The antigen-antibody complex between specific antibodies to TGE virus in the sample and the TGE antigen pre-coated in the wells is adsorbed on the plate surface and detected by peroxidase labelled anti-swine IgG. If the sample contains virus-specific antibodies, the conjugate interacts with the antigen-antibody complex, and colour develops upon addition of chromogen-substrate solution. Colour development is directly proportional to the amount of specific antibodies in the sample.

Storage:

The kit is stored at a temperature between 2 and 8°C and at not more than 80% relative humidity. The components must not be frozen.

Shelf life:

12 months.