The test system for detection of transmissive gastroenteritis virus and differentiation between TGE and porcine respiratory coronavirus using PCR

The test system is designed for detection of transmissive gastroenteritis virus (ТGE) and differentiation between TGE and porcine respiratory coronavirus (PRCV) in intestine and faeces samples from sick animals as well as infected cell cultures using PCR.

The kit contains sufficient reagents to perform 50 tests.

The assay consists of the isolation of the total RNA, reverse transcription reaction and amplification (RT-PCR) of the specific fragment using PCR, reamplification and agarose gel electrophoresis.

• набор для выделения РНК (набор 1);
• набор для выявления РНК вируса ТГС и РКВС методом PCR(набор 2);
• набор для проведения электрофореза (набор 3).

Тест-система рассчитана на проведение 50 анализов.

Total assay run time: от 8 до 16 часов (в зависимости от количества проб и используемого оборудования).

TGE virus can be detected and differentiated from PRCV using RT-PCR. cDNA is synthesized from the viral RNA, and its specific fragment is amplified millions of times, which allows visualization of the results using agarose gel electrophoresis.

PRCV is a deletion mutant of TGE virus. In the course of nested PCR, an 874-bp fragment is synthesised from TGE virus, whereas only short 192 to 253-bp fragments (depending on the strain) are synthesized from PRCV.

Since the primer binding sites are identical for both viruses, so are, accordingly, the amplification conditions.

Kit 2 is stored at a temperature between −18 and −20°С, kits 1 and 3 are stored at a temperature between 2 and 6°С.

12 months.